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Extra resources for Amino Acids in Animal Nutrition
Taurine, a metabolic derivative of cysteine, is essential for cats but it also plays a role in infant nutrition. It occurs in the nonprotein-bound form and can, in principle, be analysed in the same way as free amino acids. 1 M hydrochloric acid in an amino acid analyser. Nicolas et al. (1990) measured taurine concentrations in infant formulas and human milk following ultrafiltration also by ion-exchange chromatography. McCarthy et al. (2001) tested a taurine assay in pet food after acid hydrolysis (6 M HCl, 110°C, 16 h) and precolumn derivatization with dansyl chloride in a collaborative trial.
1996). A great advantage of this method is the rapidity and simplicity of fluorescence measurement as no prior protein hydrolysis is necessary. In vitro methods are appropriate and helpful for comparing different treatments of a feed raw material or foodstuff. Relative effects are meaningful in such tests, but absolute results concerning lysine availability are heavily influenced by matrix effects on the test system and often diverge considerably from animal experiments. Amino Acid Analysis of Feeds Data on precision and accuracy Regular collaborative trials on amino acid analysis have been conducted for many years by different organizations, and quality assurance of this kind is indeed obligatory today if a laboratory wishes to become accredited, however these results are not published.
1999) with 30% sulphosalicylic acid. Van de Merbel et al. (1995) described online amino acid monitoring in fermentation processes employing ultrafiltration for protein separation and ion exchange chromatography. There are thus many suitable variants for the removal of unwanted protein and the recovery of supplemented amino acids should always be tested in preliminary experiments. Taurine, a metabolic derivative of cysteine, is essential for cats but it also plays a role in infant nutrition. It occurs in the nonprotein-bound form and can, in principle, be analysed in the same way as free amino acids.
Amino Acids in Animal Nutrition by D_Mello